A scalable purification strategy for removal of dsRNA byproducts following IVT RNA production

Wednesday 08:00 PDT / 11:00 EDT / 16:00 BST / 17:00 CEST

Sponsor

A scalable purification strategy for removal of dsRNA byproducts following IVT RNA production

This webinar will outline a scalable and efficient affinity solution for removal of double-stranded RNA (dsRNA) from RNA produced via in vitro transcription (IVT). This includes mRNA, circular RNA, self-amplifying RNA, and trans-amplifying RNA. dsRNA byproducts are large, highly immunogenic, and cannot be removed with common bioprocessing steps such as TFF, or olig-dT affinity chromatography.

Gain insights and review case study data on a novel and scalable approach to dsRNA removal that utilizes a scavenger resin in a rapid flow-through processing step, in order to achieve a 2-3 log reduction in dsRNA levels. mRNA recoveries are typically >90%, and the flow-through operation results in simple optimization when compared to a bind/elute chromatography step.

Attend this webinar to learn about:

Common dsRNA byproducts, so as to clearly define their size, nature, and impact on RNA production via IVT
Comprehensive analytical characterization and quantification of dsRNA levels in transcribed RNA
Real-world feed data demonstrating an effective dsRNA removal process with proven 2-3 log reductions and high-yield recovery rates exceeding 90%, ensuring minimal product loss during purification steps
Streamlined, scalable purification processes that simplify workflows and accelerate production timelines

Nathaniel Clark

Downstream Scientist at Repligen

Dr. Nathaniel Clark is a Downstream Scientist at Avitide, a Repligen company. He has a PhD in biochemistry and structural biology from UMass Amherst, and moved into field of RNA as postdoc at the University of Texas. At Avitide, he uses structural biology, protein engineering, and modeling to design novel affinity resins.

in 14

Days