Advancing nucleic acid therapeutics: scalable strategies for plasmid DNA, mRNA, and LNP purification supported by rapid at-line analytics

Thursday 08:00 PST / 11:00 EST / 16:00 GMT / 17:00 CET

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Advancing nucleic acid therapeutics: scalable strategies for plasmid DNA, mRNA, and LNP purification supported by rapid at-line analytics

Efficient, reproducible production of mRNA-based medicines depends on a well-designed continuum spanning plasmid DNA (pDNA) preparation, in vitro transcription (IVT), and lipid nanoparticle (LNP) formulation. In this session, expert speakers will outline how targeted process choices at each stage can significantly improve yield, reduce impurity burden, and simplify downstream development.

Attendees will discover how lysis strategies influence pDNA recovery, what parameters most directly affect IVT reaction performance, and how capture and polishing steps can maximize mRNA purity. The speakers will highlight chromatographic approaches that enable both purification and characterization of increasingly complex LNP formulations. Collectively, these insights will provide a roadmap for developing nucleic acid processes that are scalable, robust, and aligned with regulatory expectations.

Attend this webinar to:

Learn how in-line lysis approaches compare with batch methods in maintaining plasmid quality and scalability
Explore which factors most strongly drive IVT efficiency and how they can be adjusted to minimize double stranded (dsRNA) formation
Understand the dual role of oligo dT in maximizing mRNA recovery and supporting impurity removal
Learn about novel chromatographic LNP manufacturing techniques that improve and simplify your process
Discover approaches for generating meaningful analytical data to characterize LNP formulations with confidence

Rok Sekirnik

Head of Process Development for mRNA/pDNA at Sartorius BIA Separations

Rok Sekirnik graduated with a degree in chemistry from the University of Oxford, where he also completed his PhD. He has worked in the areas of mAb, vaccine and gene therapy vector development in various roles, including Principal Scientist at Novartis and Project Manager at Batavia Biosciences. His team at BIA Separations is focused on developing high-yielding, cost-effective purification approaches for pDNA and mRNA production.

Tristan Kovačič

Project Manager, Process Analytics Development at Sartorius BIA Separations

Tristan is responsible for the development of applications for non-viral drug delivery systems, including LNPs. His expertise spans analytical development, purification, and manufacturing, with experience in biomolecule processing using monolithic columns. His experience includes development of nucleic acid-based therapeutics, as well as small-molecule process development.

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