Optimizing plasmid DNA purification: strategies for reliable and expandable processes

Tuesday 08:00 PDT / 11:00 EDT / 16:00 BST / 17:00 CEST

Sponsor

Optimizing plasmid DNA purification: strategies for reliable and expandable processes

Plasmid DNA (pDNA) quality is critical in the production of AAV, lentiviral vectors, and mRNA. This webinar will delve into the specific technical challenges faced during pDNA production, to maximize the yield and purity of the final product while maintaining compliance with regulatory standards.

Discover how tools such as Design of Experiments (DOE) and chromatographic analysis can streamline purification processes, enhance recovery rates, and ensure consistent production quality at gram scale.

Our expert speakers will discuss the latest advances and trends in pDNA production, including scalable and robust process designs that meet the growing demand for high-quality pDNA in therapeutic applications.

They will also draw on their extensive knowledge and practical experience to present case studies including real-life data on optimization of pDNA yield and quality.

This webinar will enable attendees to:

Achieve enhanced robustness and improved contaminant profile, including genomic DNA and isoform homogeneity with in-line/continuous lysis
Effectively remove most host cell RNA and proteins while concentrating multiple pDNA isoforms (OC, SC, multimers) in the eluate
Implement selective hydrophobic interaction chromatography (HIC) techniques for purity analysis to monitor the quality of pDNA throughout the production process
Ensure compliance with regulatory standards on pDNA quality

Christy Franco

Director, Commercial Operations at VGXI

Ms. Franco joined VGXI as Business Development Manager in 2014, bringing extensive experience from her role as Director of Research and Product Development at Twister Biotech. She has over 8 years of laboratory experience in gene and cell therapy, fermentation process development, and biomaterial design. Ms. Franco holds a BS in Biomedical Engineering from the University of Texas at Austin and a PhD in Bioengineering from Rice University.

Jasmina Puc

Product manager for nucleic acid at Sartorius BIA Separations

Dr Jasmina Puc earned her PhD in Biosciences from the University of Ljubljana in 2016, specializing in gene identification related to leanness in mice. She joined Sartorius BIA Separations in 2018 as a project manager, focusing on nucleic acids and developing scalable processes for DNA and RNA therapeutics using CIM technology. In March 2025, she transitioned to the business development unit as a product manager, where she continues to enhance production processes for plasmid DNA, messenger RNA, and other nucleic acid-based therapeutics.

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