Mar
24
2026
Upcoming webinar

Achieving >80-fold reduction in dsRNA from mRNA feeds using affinity chromatography

Tuesday 09:00 PDT / 12:00 EDT / 16:00 GMT / 17:00 CET
Sponsor
Achieving >80-fold reduction in dsRNA from mRNA feeds using affinity chromatography

Live30 webinars pack the latest innovations and applications into a data-rich 30-minute session.

Learn how to reduce immunogenic dsRNA byproducts generated during in vitro mRNA transcription by more than 80-fold using a validated dsRNA-specific affinity resin. Review data demonstrating that combining optimized IVT with affinity purification reduced residual dsRNA to 0.7 PPM, with the resulting mRNA demonstrated to be non-immunogenic.

This webinar will present a novel method for selective removal of immunogenic dsRNA using a validated dsRNA-specific affinity resin. Our expert speaker will review published data showing effective removal of dsRNA from mRNA feeds, with no negative impact on RNA integrity. Additionally, the purified RNA, synthesized with standard nucleotides, induced no inflammatory response in a reporter cell line assay measuring innate immune activation.

Attendees will review quantitative performance data and practical implementation considerations for integrating this scalable purification strategy into mRNA manufacturing workflows to improve purity, consistency, and safety.

Attend this webinar to:

  • Review quantitative data demonstrating >80-fold dsRNA reduction without compromising RNA integrity, even with optimised IVT processes
  • Understand how dsRNA-specific affinity chromatography selectively separates dsRNA from ssRNA to eliminate associated immunogenic responses in cell-based assays
  • Learn about key development and validation considerations for implementing dsRNA-targeted affinity resins in mRNA purification workflows
  • Gain practical insight into applying scalable affinity purification strategies to improve quality and reduce immunogenic risk in RNA vaccines and therapeutics
Nathaniel Clark, PhD
Nathaniel Clark, PhD
Senior Scientist at Repligen

Nathaniel Clark, PhD, is a Senior Scientist at Repligen. He has a doctorate in biochemistry and structural biology from UMass Amherst and studied RNA as a postdoctoral researcher at the University of Texas. At Repligen, he uses structural biology, protein engineering, and modeling to design novel affinity resins.

In 25 Days

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SPEAKERS

Nathaniel Clark, PhD
Nathaniel Clark, PhD
Senior Scientist at Repligen

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