Case study: real-time AAV titer monitoring validated against ddPCR and immunoassay

Tuesday 08:00 PDT / 11:00 EDT / 16:00 BST / 17:00 CEST

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Case study: real-time AAV titer monitoring validated against ddPCR and immunoassay

This webinar presents real-world case study data comparing a continuous, in-line titer monitoring approach against ddPCR and immunoassay across a full concentration-diafiltration-concentration workflow. Attendees will see how real-time concentration data compared against established methods at each process stage, and how continuous measurements support earlier, more informed process control decisions.

Attend this webinar to:

Review case study data showing in-line titer measurements within 9% of orthogonal methods at matched process stages, showing results within 25% across the full dataset
See how continuous monitoring detected transient events during diafiltration, such as material release from the membrane, that endpoint sampling missed
Learn how milestone sampling combined with real-time data reduced analyst-to-analyst variability to under 3%, versus higher variability with standard PCR-based testing
Gain practical considerations for applying real-time analytics to reduce development cycles and support late-stage and commercial manufacturing timelines

Paul Mania

Field Applications Scientist at Repligen

Paul Mania, Field Applications Scientist, has been with Repligen since 2017. Leveraging his training in Biological Science from Duquesne University and industry experience as a laboratory technician, he now specializes in method validation and implementation, and integrating process analytical technology (PAT) solutions as smoothly as possible. Paul enjoys working closely with pharma and biotech companies to assess their current methods and process, understand their specific requirements, and optimize their process development and QC applications to take full advantage of at-line concentration measurement and the faster accuracy of Variable Slope UV Spectroscopy.

Teva Smith

Field Applications Scientist at Repligen

Teva Smith is a Downstream Field Application Scientist at Repligen with 5+ years of experience supporting bioprocessing customers through onsite demonstrations and downstream optimization. He specializes in UF/DF, chromatography, and fill-to-finish workflows, providing post-sale support including implementation, validation, SOP development, and method transfer. He also partners with engineering on alpha/beta testing to inform R&D projects. Previously, Teva was a Product Development Research Associate at AstraZeneca, supporting simulated-use inhalation trials and cGMP data collection with R-based statistical analysis.

In 38 Days

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