Optimizing CRISPR/Cas9 cell line development with microfluidic single-cell sorting

Thursday 08:00 PDT / 11:00 EDT / 16:00 BST / 17:00 CEST

Sponsor

Optimizing CRISPR/Cas9 cell line development with microfluidic single-cell sorting

Generating validated knockout cell lines demands both precise genome editing and reliable single-cell isolation. This session presents case study data on CRISPR/Cas9 knockout generation across commercially available and immortalized primary renal epithelial cells and iPSCs, with a focus on workflow strategies that improve clonal outgrowth, viability, and throughput.

Speakers will share experimental results from the optimization and validation of ß2M knockout iPSC clones, including protein-level confirmation of successful editing. They will demonstrate how integrating advanced culture reagents with microfluidic sorting technology can reduce the time and labor associated with clonal cell line development.

Join this webinar to:

Review case study data on CRISPR/Cas9 knockout generation across renal epithelial cells and iPSCs, including ß2M knockout clones validated at the protein level
Learn strategies for improving single-cell survival, sorting accuracy, and clonal outgrowth to increase throughput and reproducibility in cell line development workflows
Apply practical guidance for overcoming bottlenecks in clonal propagation, genomic screening, and the integration of microfluidic sorting with advanced culture media
Gain practical strategies for improving single-cell survival during cloning using CEPT cocktail supplementation, and for selective sorting of fluorescently labelled, viable gene-edited cells with dual-laser fluorescence detection

Courtney J. Haycraft, PhD

Research Scientist Specialist, Department of Cell, Developmental, and Integrative Biology (CDIB) at the University of Alabama at Birmingham

Courtney J. Haycraft received her PhD from the University of Alabama at Birmingham (UAB) in in 2003. She returned to UAB in 2019 as a Senior Staff Scientist in the research lab of Bradley K. Yoder, PhD. The Yoder lab focuses on the function of primary cilia in development and renal cyst formation using cell and rodent models.

Bhamini Purandare, MSc

Senior Scientist, Application Science, at R&D Systems by Bio-Techne

Bhamini Purandare is a Senior Scientist at Bio-Techne with over 15 years of experience in cell biology, immunology, and gene editing. She leads technical strategies for innovative workflows, including the use of the Pala microfluidic cell sorter for gentle single-cell dispensing and iPSC genome engineering. Bhamini’s expertise includes assay development, T cell engineering, and clonal cell line generation, enabling advancements in both research and therapeutic applications. She holds a Master’s degree in Biological Science and an M.Sc. in Medical Microbiology with numerous contributions to translational research and cross-functional collaborations.

In 43 Days

You have registered for this webinar