Multiple CQAs from a single dataset: advancing lentiviral vector-based cell & gene therapy

Wednesday 08:00 PST / 11:00 EST / 16:00 GMT / 17:00 CET

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Multiple CQAs from a single dataset: advancing lentiviral vector-based cell & gene therapy

As developers of lentiviral vector (LVV)-based cell and gene therapies continue to innovate, the demand for high-quality LVV is expected to rise significantly. This growing need necessitates advanced characterization techniques that can enable process development, providing improved performance, efficacy, and safety.

In this webinar, we will discuss the qualitative and quantitative capabilities of cryo-transmission electron microscopy (cryo-TEM) imaging, highlighting its unparalleled precision in characterizing the morphology, integrity, and composition of viral vectors, and its role in ensuring the high quality and efficacy of LVVs for therapeutic applications.

We will cover the entire workflow, from sample preparation and vitrification to automated image analysis using advanced Amira 3D software.

Attend this webinar to understand:

How cryo-TEM enables a detailed analysis of LVV critical quality attributes (CQAs) such as particle size, shape, surface area, and the presence of impurities
The process of vitrification and its importance in preserving sample integrity
How to achieve multiple CQA determination from a single cryo-TEM dataset
The identification and quantification of LVV impurities and their impact on gene therapy efficacy

Tilak Gupta

Global Product Marketing Manager, Biologics at Thermo Fisher Scientific

Tilak Gupta received his Ph.D. in Biochemistry from the Max Planck Institute of Biochemistry, followed by postdoctoral research on extracellular vesicle characterization using cryo-TEM. He is passionate about driving the discovery and development of next-generation therapies—including antibodies, vaccines, and cell and gene therapies—by enabling researchers to accelerate development timelines through structural insights from cryo-EM. Combining a strong scientific background with industry experience in research, applications, and marketing, Tilak brings both technical expertise and strategic perspective to help biopharma innovators leverage advanced imaging technologies for therapeutic development.

Rengarajan Pelapur

Sr. Manager, Global Product Applications at Thermo Fisher Scientific

Rengarajan (Reng) Pelapur received his Ph.D. in Computer Science focused on Computer Vision, Image Processing and Machine Learning from the University of Missouri, in 2016. Rengarajan’s research interests include feature detection, object tracking, segmentation in large 2D/3D data across various domains such as aerial, biomedical, life sciences, material sciences and semiconductors. Rengarajan uses his programming, machine learning and image processing expertise to assist customers solve large data processing and visualization problems.

Jana Škařupová

Application Scientist at Thermo Fisher Scientific

Jana received her Masters Degree in Applied Biosciences from Anglia Ruskin University. Originally a biomedical scientist specializing in cell and tissue cultures, Jana’s work included structural studies of receptors triggering inflammation pathways, as well as expression of recombinant biologics. Jana is currently an Application Scientist at Thermo Fisher Scientific with a focus on the preparation of cryo-EM specimens.

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