Adenylator DNA/RNA 5΄-Adenylation Kit: clean, efficient enzymatic adenylation of DNA and RNA

Nucleic Acid Insights 2025; 2(9), 197

DOI: 10.18609/nuc.2025.028

Published: 14 October
FastFacts
Lucas Onder


 

 

Adenylated oligonucleotides are essential for ligation-based applications, adapter ligation for next-generation sequencing (NGS), preparation of linkers for ligation-based cloning, and protection of DNA/RNA 5′-ends from exonuclease degradation. 

This 7.5-minute video presentation, accompanied by a poster summary, describes data on a novel high-specificity adenylation kit that enables efficient, artifact-free oligonucleotide preparation for ligation and NGS workflows. 

Watch the video or view the poster to learn about: 

  • An advanced adenylation kit that efficiently adenylates DNA and RNA oligonucleotides for ligation workflows 
  • Case studies comparing performance of a novel adenylation kit to other widely-used enzymes for achieving high-specificity products with fewer byproducts 
  • Data showing how adenylated oligonucleotides are resistant to exonuclease degradation, and deliver a 3-fold higher yield per reaction than Mth RNA Ligase
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