Precise mRNA purity assessment for accelerated therapeutic development and release testing

Nucleic Acid Insights 2025; 2(5), 41

DOI: 10.18609/nuc.2025.009

Published: 4 June
Webinar Digest
Paul Mania, Joe Ferraiolo


 Traditional fixed-pathlength UV-Vis spectroscopy for nucleic acid analytics has limitations, including the need for sample dilution, which introduces variability and increased preparation time. Variable pathlength technology (VPT) is a UV-Vis-based spectroscopy technique that enables the measurement of multiple absorbance values through variable pathlengths, improving the accuracy and efficiency of nucleic acid analytics. Case studies explored in this poster demonstrate VPT’s effectiveness in measuring pDNA and mRNA purity, highlighting its role in streamlining processes and enhancing product development.

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