From EV to LV: Scalable purification using membrane chromatography

Wednesday 08:00 PDT / 11:00 EDT / 16:00 BST / 17:00 CEST

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From EV to LV: Scalable purification using membrane chromatography

Extracellular vesicles (EVs) and lentiviral vectors (LVs) are transforming the future of cell and gene therapy, but their fragility makes purification uniquely difficult. Both modalities are highly sensitive to shear stress, pH shifts, and osmotic pressure, and conventional purification methods often fall short.

This webinar shows how weak anion-exchange (AEX) membrane chromatography offers a gentler, more scalable route for both modalities. The speakers will compare the approach to an established strong AEX workflow and present new lentiviral vector data on functional recovery, residence time, and protein depletion during loading. In addition, the webinar will also show application of weak anion exchange chromatography for effective purification of EVs.

Attend this webinar to:

Understand why EVs and LVs require purification methods matched to their fragility, rather than workflows adapted from other modalities
See how weak AEX membranes improve exosome recovery compared with strong AEX by ~14%
Learn how a purpose-designed AEX membrane delivered around 3.5-fold higher functional LV recovery at large scale compared to a standard Q-membrane process, regardless of residence time
Discover how weak AEX loading removes up to 90% of host cell proteins early in the process

Franziska Bollmann

Senior Program Manager at Sartorius

Dr. Franziska Bollmann, PMP®, is a Senior Program Manager for Separation Technologies at Sartorius, where she leads a strategic portfolio of over 20 cross-functional projects and drives global collaborations with industry and academic partners. Since joining Sartorius in 2017, she has built extensive expertise in downstream bioprocessing and viral vector applications, with a strong focus on translating customer needs and market insights into product strategy, innovation initiatives, and portfolio decisions. In the past ten years in the biotech industry, she has worked at the interface of science and strategic marketing, supporting product positioning, commercialization, and customer-centric innovation through strategic collaborations, scientific communications, and technical content development.

Swapnil Chaudhari

Global Product Manager at Sartorius

Dr Swapnil Chaudhari is a strategic product and business leader with extensive experience in the biopharmaceutical and biotechnology industry. He currently leads the global Sartobind® membrane chromatography consumables portfolio at Sartorius, with a focus on product strategy, portfolio management, commercialization, and growth in advanced bioprocessing and cell and gene therapy markets.

With a PhD in Biotechnology and a background spanning cell culture media, upstream and downstream bioprocess development, and product management, Swapnil combines deep technical expertise with strong commercial and strategic capabilities. His experience includes leading cross-functional teams, developing go-to-market strategies, driving customer collaborations, commercial leadership and translating market and customer insights into innovative product and business opportunities.

In 54 Days

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