Balancing the tradeoffs in IVT: enzyme engineering, reaction optimization, and dsRNA control for safe, scalable RNA production
Vaccine Insights 2025; 4(8), 275–278
DOI: 10.18609/vac.2025.041
Published: 6 November
Viewpoint
Kaixi Zhao, Emily Wen
“The growing demand for RNA-based vaccines and therapeutics has exposed critical trade-offs in current in vitro transcription (IVT) workflows. To ensure safe and scalable RNA production, manufacturers must balance enzyme engineering, reaction optimization, and dsRNA control to improve efficiency without compromising quality.”