Accelerate UF/DF of nucleic acids with real-time monitoring and automated process control 

Join this webinar to explore data from four key case studies demonstrating how real-time, in-line concentration measurement enables precise control of UF/DF workflows.

Real-world data will be presented detailing runtime comparisons showing significant time savings over traditional workflows and achieving ≤5% deviation from target concentration endpoints.

Case study 1: crude DNA determination

• The concentration of double stranded DNA was monitored during TFF, and the concentration used as an endpoint control to dictate process mode changes
• Learn how to optimize your diafiltration step when impurities are present in your sample

Case study 2: mRNA determination 1

• High concentrations of mRNA were monitored and used as an endpoint control during TFF
• Learn how to measure above 11 mg/ml with accuracy and precision, with no need for offline readings, even with samples at very high optical densities

Case study 3: mRNA determination 2

• TFF process control of mRNA
• Learn how to determine gel polarization of molecules on a membrane, with no need for dilutions or other forms of sample manipulation

Case study 4: encapsulated mRNA concentration

• The concentration of total mRNA that has been encapsulated by a lipid nanoparticle was determined
• Learn how to achieve accurate, repeatable, and reproducible measurements of mRNA encapsulated in LNPs

Attend this webinar to learn:

• How real-time in-line concentration measurement supports precise control of UF/DF workflows
• Approaches for improving consistency and accuracy when concentrating or buffer-exchanging mRNA, plasmid DNA, or genomic DNA
• How to implement automation for nucleic acid TFF without disrupting existing lab-scale setups

Nigel Herbert

Senior Bioanalytics Applications Specialist at Repligen

Nigel Herbert stands as a dedicated advocate for our customers' success. With a Bachelor of Science degree in Biomaterials Engineering and a minor in Chemistry from Alfred University, Nigel brings a wealth of skills to his role as a Senior Bioanalytics Applications Specialist at Repligen.

Since joining our team in 2019, Nigel has been on the forefront of advancing our customers' analytical methods. He specializes in UV-Vis spectroscopy, analytical processes, and method validation, offering invaluable support to ensure the highest standards of precision and reliability.

One of Nigel's remarkable achievements is his collaborative spirit, resulting in numerous published application notes. Through close partnerships with our customers, he has made significant strides in solving complex challenges and driving innovation in the biotechnology field. Nigel Herbert's unwavering commitment to enhancing your analytical capabilities exemplifies Repligen's dedication to your success.

Watch now

SPEAKERS

Nigel Herbert

Senior Bioanalytics Applications Specialist at Repligen

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