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Lentiviruses are a commonly used vector for both CAR-T cell therapy and ex vivo gene therapy. Vector purification can be highly challenging due to the low stability of these enveloped viruses, which are sensitive to low pH, high salt, temperature, shear forces, and other factors. To maximize physical and infectious titer, it is important to carefully finetune the conditions of each downstream step with these sensitivities in mind.
Cytiva has continued to refine its downstream process by optimizing capture from clarified feeds using weak anion exchange with Capto™ DEAE resin, followed by a Capto Core 700 resin polishing evaluation with different buffer pH values and flow rates.
In this webinar, we show that using pH 7.0 significantly improved infectious recovery without impacting physical recovery. We also show that residence times down to 0.7 minutes can be used. After the polishing step, total DNA and protein were below the level of quantitation for all conditions.
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