May
31
2022
On demand

Monitoring of circulating CAR T cells

Sponsor
Monitoring of circulating CAR T cells

The expansion and persistence of CAR T cells within patients is the key to a successful treatment. Therefore, monitoring of CD19 CAR T cells following infusion is of utmost interest. The CAR detection reagent is the most critical reagent for CAR T cell flow cytometry assays. When selecting a strategy for CAR detection, the impact of the reagent on the assay specificity and reliability must be taken into consideration. Join Dr. Sabine Hünecke and Dr. Melanie Bremm from University Hospital Frankfurt and Dr. Annika Graband from Miltenyi Biotec to learn about

  • Different strategies for CAR detection by flow cytometry
  • Validation of a CAR T persistence assay
  • Achieving comparable results at different sites (external quality assessments, EQA in collaboration with the Karolinska Institute)

Annika Graband

Product Manager, Cell Analysis Reagents, Miltenyi Biotec

Annika is Miltenyi Biotec’s Product Manager responsible for the cell and gene therapy reagents portfolio, including CAR detection reagents and StainExpress™ dry antibody cocktails. During the past year, she has been working together with R&D to advance the development of tools that enable highly reliable and standardized cell and gene therapy analytics.

Dr. Sabine Hünecke

Head of Laboratory, Stem Cell Transplantation and Immunotherapy, Department for Children and Adolescents, University Hospital of Frankfurt

Sabine Hünecke received her Ph.D. in 2009 at the Goethe-Universität in Frankfurt, Germany, in the fields of stem cell transplantation and immunology. After her time as post-doc, in which she worked on optimizing cellular therapies their quality control and immune monitoring in patients, she became head of the laboratory for stem cell transplantation and immune therapies in 2012 at the University Hospital in Frankfurt. There, Sabine continues to work on the research of cellular immunotherapies, the immune monitoring of transplanted patients and the further development of cell purification methods and flow cytometric analysis for the characterization of stem cell transplants and cellular preparations.

Dr. Melanie Bremm

Deputy Head of Laboratory, Stem Cell Transplantation and Immunotherapy, Department for Children and Adolescents, University Hospital of Frankfurt 

Melanie Bremm performed her Ph.D. projects at the Goethe-Universität in Frankfurt, Germany, and then continued her Postdoc studies at the laboratories for stem cell transplantation and immunotherapies at the University Hospital in Frankfurt until 2012. Melanie took over the role as deputy head in the same laboratories in 2013 then and - as an example - since then is working on developing new cellular immunotherapies, assessing the cellular immune status in patients using flow cytometry and ensuring the production & quality control of stem cell preparations and immunotherapies under GMP.

SPEAKERS

Annika Graband
Annika Graband
Product Manager, Cell Analysis Reagents at Miltenyi Biotec
Dr. Sabine Hünecke
Dr. Sabine Hünecke
Head of Laboratory, Stem Cell Transplantation and Immunotherapy at University Hospital Frankfurt
Dr. Melanie Bremm
Dr. Melanie Bremm
Deputy Head of Laboratory, Stem Cell Transplantation and Immunotherapy at University Hospital Frankfurt

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