Rapid, simple purification of AAV using low-speed centrifugation
Cell and Gene Therapy Insights 2026; 12(8), 866
10.18609/cgti.2026.109
Published: 30 September
Tech Showcase
The IsoTag™ AAV Rapid Screening Kit combines affinity and size-based purification into a single low-speed centrifugation protocol, while delivering the ideal combination of recovery and impurity clearance that screening workflows require. This helps scientists purify AAV samples quickly and simply to help facilitate confident decisions about therapeutic candidates and preclinical programs.
Watch the full Tech Showcase video to learn how the IsoTag™ AAV Rapid Screening Kits can help scientists:
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Purify AAV in 2–3 hours with no specialized equipment or extensive training
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Use one single reagent and process across AAV serotypes 1, 2, 3, 6, 8, 9, and PHP.B
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Achieve consistent capture and elution performance regardless of user experience level thanks to a simple, centrifuge-based protocol
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Obtain 77%–95% capture and >60% elution with >99% clearance of host cell protein, host cell DNA, and residual IsoTag™ reagent for a variety of AAV serotypes
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Purify starting volumes ranging from 1 mL to 40 mL, using the same protocol
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Utilize workflows that fit their individual research needs by offering consistent performance across both clarified and unclarified lysate and the ability to concentrate up to 10X during elution
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