Scaling cell therapy engineering with microfluidic single cell dispensing and automated capillary-based western immunoassays
Cell and Gene Therapy Insights 2026; 12(7), 741–754
10.18609/cgti.2026.090
Published: 18 August
Innovator Insight
What you will learn01How combining fluorescence-based single cell dispensing (Pala) with automated capillary western immunoassay (Leo) addresses two major bottlenecks in iPSC CRISPR engineering: single cell cloning and functional knockout confirmation02Why IFNγ-induced surface β2M expression improves fluorescence-based enrichment of edited clones, increasing knockout recovery to 62% versus 40% in untreated cultures03How Simple Western analysis discriminates partial from complete β2M knockout and detects truncated or altered protein products missed by flow cytometry, even when all clones appear negative by surface staining04Why whole cell protein analysis is a valuable orthogonal method to surface-expression screening, which can overestimate the true frequency of functional knockout05How Pala's gentle sorting supports high monoclonal colony yields (>30 colonies/96-well plate) while preserving pluripotency, with all clones exceeding 70% co-expression of NANOG, OCT4, and SSEA406How this combined workflow provides a scalable, reproducible strategy for generating and validating engineered iPSC-derived cell therapies and disease modelsKey interests
What you will learn
01
How combining fluorescence-based single cell dispensing (Pala) with automated capillary western immunoassay (Leo) addresses two major bottlenecks in iPSC CRISPR engineering: single cell cloning and functional knockout confirmation
02
Why IFNγ-induced surface β2M expression improves fluorescence-based enrichment of edited clones, increasing knockout recovery to 62% versus 40% in untreated cultures
03
How Simple Western analysis discriminates partial from complete β2M knockout and detects truncated or altered protein products missed by flow cytometry, even when all clones appear negative by surface staining
04
Why whole cell protein analysis is a valuable orthogonal method to surface-expression screening, which can overestimate the true frequency of functional knockout
05
How Pala's gentle sorting supports high monoclonal colony yields (>30 colonies/96-well plate) while preserving pluripotency, with all clones exceeding 70% co-expression of NANOG, OCT4, and SSEA4
06
How this combined workflow provides a scalable, reproducible strategy for generating and validating engineered iPSC-derived cell therapies and disease models
Key interests