Novel resin design enables efficient enrichment of full AAV capsids by anion exchange chromatography

Key findings

• The HiPer^™ QA resin achieves high salt tolerance and resolution of empty and full AAV capsids
• The purpose-built QA resin demonstrates lot-to-lot consistency, resulting in improved development costs
• Resin performance remains consistent regardless of flow rate, while also maintaining AAV separation efficiency even with 15 seconds residence time
• The HiPer QA resin enables efficient separation of AAV9 variants under high-conductivity buffer conditions

Summary

• The micrometer-scale pore size of the resin improves pore accessibility for large biomolecules such as AAVs
• The purpose-built QA resin enables rapid polishing of different AAV serotypes, minimizing the residence time and reducing the risk of capsid degradation
• Purification conditions can be applied across multiple lots of resin, helping manufacturers manage development costs

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