Downstream Processing 2025

Optimizing your TFF process for AAV manufacturing: a guide to evaluating hollow fibers versus flat sheets

Cell & Gene Therapy Insights 2025; 11(7), 823–829

10.18609/cgti.2025.092

Published: 29 July
Interactive Insights


A typical AAV manufacturing process involves two tangential flow filtration (TFF) steps: the pre-affinity step, which occurs toward the beginning of the process, and the final formulation step, which takes place toward the end. Two commonly used TFF formats are hollow fibers and flat sheets - but which will be best suited to a specific target molecule or application?

Explore this interactive visual guide which provides a step-by-step method for evaluating different TFF formats and optimizing your processes, presented by AAV manufacturing expert Luke McCarney.