Efficient AAV purification: resin reuse and scalable polish method

Pan-affinity resins, such as the Thermo Scientific™ POROS™ CaptureSelect™ AAVX bind to a broad range of AAV serotypes and offer efficient and scalable AAV production. These resins have allowed the purification procedure to be simplified to a single step, thereby reducing process development time and cost. However, affinity resins can be expensive as they are often discarded after a single use, making resin reuse an important objective in the effort to lower production costs. A further key challenge in AAV purification is the large-scale removal of empty and partially full capsids. The wide variety of both available resins and process conditions can result in significant variations in capsid enrichment levels. This article explores the application of the POROS CaptureSelect AAVX affinity resin reuse protocols and assesses the performance of the Thermo Scientific™ POROS™ 50 HQ anion exchanger resin in optimizing AAV purification.

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