Strategies to accelerate AAV chromatography process development

Cell & Gene Therapy Insights 2023; 9(9), 1271–1285

DOI: 10.18609/cgti.2023.164

Published: 8 November 2023
Innovator Insight
Kelley Kearns

Following rapid growth in the gene therapy sector, the viral vector manufacturing industry is currently facing the key challenge of enabling and optimizing large-scale AAV production. Cleanable, reusable affinity resins can help to reduce costs in downstream purification of AAV vectors. This article provides a technical overview of rapid process development for an AAV capture step, using AVIPure® AAV affinity resins in two case studies. A high-throughput design of experiments framework will be shared on how to effectively identify desirable conditions for AAV chromatography.