Downstream bioprocessing 2022

Improved lentivirus recovery by weak anion exchange capture

Cell & Gene Therapy Insights 2022; 8(9), 1513–1520

DOI: 10.18609/cgti.2022.222

Published: 16 December 2022
Innovator Insight
Lisa Marx

Lentivirus is one of the most efficient gene transfer vectors for integrating a gene of interest into the target cell DNA. The purification of lentiviral vectors is challenging due to the low stability of this enveloped virus that is sensitive to low pH, high salt, temperature, and shear forces. Typically, lentivirus purification is carried out using anion exchange capture chromatography, but with low recoveries. Here, weak anion exchange capture is identified as a method for improved lentivirus recovery using the CaptoTM DEAE resin.