Standardizing CAR-T quality control: dPCR-based approaches for reliable VCN and RCL testing
Sponsor
Live30 webinars pack the latest innovations and applications into a data-rich 30-minute session.
CAR-T programs depend on precise, reproducible analytical control. Yet, vector copy number (VCN) and replication-component lentivirus (RCL) measurements often exhibit variability across platforms and workflows. This webinar will show how to establish consistent QC strategies using digital PCR (dPCR), compare results across common manufacturing systems, and interpret assay performance with real-world data.
Attendees will see comparative VCN and RCL readouts from three CAR-T manufacturing approaches and gain insights into threshold setting, target selection and the impact of restriction digestion on assay performance and data interpretation. The session will also outline a comprehensive Critical Quality Attribute (CQA) framework and corresponding assays to support safe, reproducible and scalable CAR-T development.
Attend this webinar to:
- Learn how to design and implement QC workflows for CAR-T across safety, viability, quantity, potency and immune phenotype, with clear guidance on what to measure and which analytical methods to apply
- Explore practical dPCR frameworks for VCN and RCL measurements, including target selection, the rationale for including a restriction digest and how standardization improves VCN estimates
- Compare three manufacturing approaches and discuss reproducibility, operational trade-offs and data interpretation across platforms
You have registered for this webinar
SPEAKERS
You might also like
Safety assays for quality control of ATMPs: improved mycoplasma detection for cell-based biotherapeutics
Achieving high yield and purity in lentiviral production using dPCR and IEX membranes
Combining CRISPR and transposon-based technologies for improved sdAb-based CAR-T therapies
